FastQCFastQC Report
Wed 6 Jul 2016
506683_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506683_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128661
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTG50.0012304022572.918235
CAGAGTA50.0012304022572.918235
TCCTATT100.0049203471286.459235
TCTATGG100.0049203471286.459235
GGGGGGG2000.009977908128.6459235
TCATATG350.001414416678.426072
TCCGTAG550.00227428569.562294160-164
GTACTTG806.4626976E-445.7485431
CCCCTGG950.001505475638.525091
TTCCGGA1100.00309110133.2716672
ACCCCCA1100.00309110133.2716676
GCGGGGG4000.0019340232.161476235
GAGAAGG1350.00841357327.163033230-234
CTGGACC3101.9337313E-623.6121524
TATCGTC1050.003864140720.513397205-209
GGCCAGT3450.002516514615.9125371