FastQCFastQC Report
Wed 6 Jul 2016
506683_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506683_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128661
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTAGT450.00209898570.99964
GTTCTGA600.006559734753.249711
GTCATAT650.00900138449.153581
TCCAGAA650.00900138449.153582
GCCCTAT650.00900138449.153581
CAACACA1255.3503914E-542.5997664
TAGGCGT1150.001825359937.0432784
TGTAGGC1200.002249438535.4998052
ATACAGC1250.002748063834.079816
GCCAAAT1450.005682688731.602236235
TCCAACA1450.005682688729.3791482
GGGCTGT2059.7639934E-425.9754687
CCCAGGG2350.002163158722.659452
TGATGGG2500.003098216121.2998833
CAGGGCA2700.00483849419.7221154
ACAACAC3000.0088888117.74993
ACACCGT4050.002663521315.7776916
CACACCG6300.003574426311.8332695
GACACCG8402.0281054E-411.4106525
AGCACCG7700.001259423811.0648755