FastQCFastQC Report
Wed 6 Jul 2016
505907_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505907_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239071
Sequences flagged as poor quality0
Sequence length50-241
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2252.0987354E-631.251471
TACCCTG1400.006381041428.700335
TATGGGC1450.007573523627.7106653
GTAATGC1857.562509E-427.1489623
TTGGGCC2451.3432822E-424.6002834
GTATAAC2050.001376519124.5002841
TATACTG2901.5094349E-524.2468325
TATAGTG3001.9623496E-523.4386045
ACTATTC2602.0098868E-423.1810383
GTGCGTG2702.5950218E-422.3224819
GCACAAC2250.00236581322.322486
ATTATGC2400.003440520520.9273243
GGTTTAA2904.2058635E-420.7829991
GTGGTAT3106.592919E-419.442161
CTATACT2650.00610408918.9530494
TCCTGGA2700.006799649418.6020682
TATTAGT3600.001798308816.7418612
GTCCAGG3600.001798308816.7418611
CTATATA6058.8574416E-716.6034984
GTATTAT4353.4051653E-416.1645551