FastQCFastQC Report
Wed 6 Jul 2016
505907_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505907_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239071
Sequences flagged as poor quality0
Sequence length50-241
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGGG1008.156818E-443.6519137
ACTAGAG1050.001037085541.573253
GTGTGAA1901.3482431E-534.462041
GTATATA5650.028.9725071
CCTCTAA2058.4891624E-426.6170221
ATAGTGC1650.00949443626.4557067
AATCCCA2350.001881765323.2191035
GTCTTTA2400.00212681122.7353731
ATATCAG3402.7212907E-522.4678975
GACAACA3457.7538326E-418.9790947
ATAAGCC2900.00636634218.815483
GTATAAG2950.007026496318.4965741
TGTAAGA2950.007026496318.4965742
ATACCAT2950.007026496318.4965746
ACTACTG3000.007741753518.1882973
CTTGATC3050.00851561217.890133
CCCCAGG3050.00851561217.890131
TTATACA3750.001357121317.4607664
ATTATAC4401.974201E-417.3615573
TATACAA5005.229298E-415.278175