FastQCFastQC Report
Wed 6 Jul 2016
505137_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505137_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126215
Sequences flagged as poor quality0
Sequence length50-241
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGG100.009114855945.7262235
GTGCTTA400.004701852166.12245225-229
GCTTTGG1250.0061663375151.31618235
GCGATAG1450.009609957130.44499235
GTATACG308.07924E-490.301111
GTTGTGT500.006124828554.1806683
CCTTATC550.00892786649.2551548
TAGACCG550.00892786649.2551545
TCACGGG1201.1120918E-437.625463
ACCGACT1301.780782E-434.7311978
ACCGTCC1150.00409836931.4090828
ACCACCC1752.7954058E-530.960388
TGTTCAC1200.00504701130.100375
GGGGGGG1350.002095480722.790178220-224
GACCGTC2050.00255112222.024667
CAGATAG1150.004677211419.846916205-209
ATTACAT500.00807793418.0602215-19
CAGCACC5851.8776409E-615.4360874
TGCGCTG5101.570145E-414.1648815
CCAGCAC5350.003615089511.8150983