FastQCFastQC Report
Wed 6 Jul 2016
505137_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505137_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126215
Sequences flagged as poor quality0
Sequence length50-241
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGA400.001197502781.783734
CTATAGA500.002902282665.426984
TCTAGAA500.002902282665.426982
TCCCTGA550.004233723559.4790731
TGTATGC600.00597432754.522482
TATAGAT650.00819879150.3284455
GTTCTAC1100.001306350639.6527141
GGGTGAA1200.00200308136.348321
CCAGAAC1652.3859226E-433.043933
GGGAGTA350.00595401229.116726190-194
AACTGCG2852.1388542E-422.9568377
GTTGGGC2750.004692681619.8263573
TTGACTG650.00891853517.75239135-139
GTGCAGG4250.003137301215.3945841
AGGTCCA1456.296867E-412.03254835-39