FastQCFastQC Report
Wed 6 Jul 2016
504782_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504782_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195304
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAAT350.004194061172.70079225-229
GACCATA653.857895E-4108.71152215-219
TAATTAC359.718573E-6106.821592
TATAGGG307.050564E-493.46892
CTAATTA307.050564E-493.46891
GGGGGGG4300.00112658182.69923235
GTTCTAG450.003523923562.3125951
CTTCAGA500.00534814256.0813332
GTAACCT500.00534814256.0813333
GTCATAG1250.00521290129.9100461
ACATCTC1250.00521290129.9100466
GGACAGT1350.007591789627.6944876
GCCCGTT3650.001555754823.975197230-234
ACTGGAC2050.00209208922.797293
GTACTGG2100.002406535722.25451
GAAAAAG1150.004264717514.713539175-179
TGGACCG4608.983214E-414.2235275
CCGTCCG5750.004804068211.3788219
GCACCGT6600.001037695311.3295636
CACCGTG7050.001799821610.60647