FastQCFastQC Report
Wed 6 Jul 2016
504782_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504782_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195304
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG650.00909818749.030251
CCTCAGT650.00909818749.030252
CGTGAGA1100.001487400638.6298942
CTAACCG1500.00679752328.3285874
TAACCGG2500.00314946221.246445
GGACGGT3104.5177547E-420.5610716
CAAGACG4051.2847308E-418.361124
CCTGGAC4201.6972664E-417.7053683
TGGGTGT3050.0099367117.4151154
AACGGTG3700.00148413817.2268435
ACTGGGC4200.00345976515.176038
CGGTACC4650.00679983413.7073814
CTGCACC5750.001841551512.9326174
ACTTGTC1400.007039617310.81651175-79
AAAAAAA3700.0053633768.6134217
CCGCGCG14700.0040923137.22668089
TCCGGAC3350.00542323067.0265245145-149