FastQCFastQC Report
Wed 6 Jul 2016
504302_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504302_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275796
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT1050.0010522247273.5275235
TTAGCGA1200.0015694493239.33656235
TTGATTA1250.0017734593229.76308235
TGTCTGG1300.0019943845220.92606235
GGTAGAA1350.0022328922212.74362235
GTTATAC254.4438962E-4104.986943
AATTATA450.00458065758.3260842
GTTATAA500.006949879752.493471
GAATAAG704.1808834E-449.9937861
GTAGAGA808.0742716E-443.744561
TATAGCC950.001879693136.8375243
TATACCG950.001879693136.8375245
GCTCTAT1050.003071098433.329191
AATACAC1150.00479419730.4309983
CCTATGG1454.0818084E-430.1686613
TACACGG1556.0311146E-428.2222985
TAGGCCG3300.002532086327.303991230-234
CATAAAT500.00840061127.135466180-184
GCCTGTC3251.0549406E-418.8438117
CAACCTC3150.001855786316.6645954