FastQCFastQC Report
Wed 6 Jul 2016
504302_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504302_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275796
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTAT550.00586974254.783763
ATTATAT959.5376186E-442.289221
TATTGAC1205.9600956E-541.848712
TATGGGT1050.001560187538.2616772
TGCAGTA1806.451285E-427.899142
GTAGTAC1806.451285E-427.899141
GCGTAAT1500.00894407126.7831751
CAGTACT1950.001029636725.7530524
GTAGAAC2450.003881811220.4973263
GGTGTCT2550.004893204719.693511
GCAGTAC4109.791149E-619.5974453
CAGTACG3157.3533534E-419.1308384
AGTACGG2650.006111424418.9503575
ATACGGC3350.00111199317.9886973
CTGCGGA5250.00141878213.3915869
GTCTTGG4500.00794438813.3915861
AGCGTAT1500.00868497613.216642215-219
GACTGCG4650.00986534112.95959957
TCAACGG5750.002811339712.2271013
CCGTGCC7850.002425008210.2356079