FastQCFastQC Report
Wed 6 Jul 2016
503710_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename503710_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163393
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAT52.6440498E-45537.707235
CATTTAA100.00105749522768.8535235
AGTCTAT200.0042289831384.4268235
TCAAATA250.00660700721107.5414235
GCAGGGG2250.0014127897246.12032235
TCTGAAC450.002655474201.03888220-224
GTATTAA100.004577563167.80931
CTACAGA300.0010823183.9046551
GATTATC350.002194065870.22027215-219
CGTCTAT850.00787208250.839203225-229
CTCAAGG2000.0078866850.762314230-234
TACAGCT705.13276E-447.945527
CACTGAA900.001767987937.290962
GTACTGG1650.001107484325.4256531
CAAGCCA1850.002155958722.6769319
AGGTCCA2050.003911226520.464555
ACCTGTT2300.007605663518.2401437
CCATGCC3600.0082831048.820559185-189