FastQCFastQC Report
Wed 6 Jul 2016
503710_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename503710_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163393
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATG150.0072119087144.144681
ACATAGT500.003004792164.8651051
ATACTGG650.00848753449.8962366
GTATGCG1753.5489522E-430.8881457
ACCCTGA1956.686925E-427.7201296
GTCTGGG2400.002243236922.5226081
CTACACC2550.00318944121.1977464
CCATCAC4000.00222154516.2162763
GGACCGC6007.474544E-616.2162766
TGGACCG4753.7936284E-415.9317795
GCACCGT6702.1254338E-514.5220386
ACCGTGC6000.002220432512.6126598
GCCCTGG6700.005070566711.2949191
ACACCGC7950.001454626310.8788446
ACCGCGC10951.8470116E-49.8729238
CACCGCG18155.455996E-57.7433097