FastQCFastQC Report
Wed 6 Jul 2016
502836_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502836_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191570
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGT100.005186761254.1908235
CTGTCTT704.6779553E-4358.34024235
TATAGAC350.001346931979.412912
TGGCGGG6600.004916697357.008675235
GTAATGG703.1486276E-452.941941
CAGAGGG550.00807316850.535494
TAGTCCG1150.003619149832.225535
AACATGT1300.00961162227.774942230-234
ATCTACC1350.00792517527.4513762
GGGGGGG4001.5525075E-824.357397230-234
CTACACC2600.00871281717.8169984
TGCACAC2600.00871281717.8169982
ACAGCGC5050.001940926312.8423528
CACCACC13206.372415E-47.72069934
CAGCGCG13650.00752040466.7874289