FastQCFastQC Report
Wed 6 Jul 2016
502836_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502836_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191570
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTTA151.2611318E-4556.37854235
TATATAG150.007312032143.487873
CACATGA1153.829137E-4108.856674235
CCTCTCA600.006299336469.54732235
TAAAGAG500.0030604864.5695345
TACAAGG500.0030604864.5695343
TGTAGAT1100.0013956839.1330532
TACTCCG1300.003168398733.1125835
TTCATGA1450.005406802529.6871432
GTACATG1500.006380915628.6975731
CAAGATG2651.4320159E-424.3658644
ACGGACC2300.001799746623.394763
CGTGTCC2952.9610452E-421.887988
CAAGGCG5400.001040152513.9502094
ATGTCGG7050.00766975810.68526657
ACACCGC8350.002277064610.3105056
CCAGCAC12350.00627405487.84245443
CTGCCGA3900.00485195737.107161210-214