FastQCFastQC Report
Wed 6 Jul 2016
502749_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502749_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178707
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGC359.388781E-6107.561273
CATTAGG400.002149433470.587092
CTCCTTA400.002149433470.587091
TTAGGCA450.003428413562.7440764
GTAGTGA500.005203343556.469671
CAGTACA900.001005529441.8293844
ATTGGAC950.001311851339.627846
GTGTACA1050.0021447835.853761
GGCTAGG1200.004126661531.372041
ATATTCG1250.005038819330.1171571
CGGGGGG3900.001559853828.942608235
AAACCCA1350.007338715327.8862575
GGGCTGC5751.024735E-616.3680216
CCAGACC4050.00607096313.9431284
CTCCAGC4300.00902277613.1324821
TGCTGGA5450.00305513412.0883082
GCTGCAG6200.00798937910.6260138