FastQCFastQC Report
Wed 6 Jul 2016
502749_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502749_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178707
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACAA150.0030135587192.96198215-219
CACTAGG500.0037283308179.56999235
TTATAGT150.007408297142.85884
AACAGTC600.00640906953.5720527
TACTGAC956.930893E-445.1133042
GTATAGG1008.922534E-442.857646
GATATGC1300.003236767832.9674151
GCCCATA1550.007649750427.6500911
CCTTGGG2200.001425026824.3509312
CCGAGTG2650.004199691620.2158669
CCGGGTA3550.00106141218.108869
GTGTCCG4100.00278228115.6796241
GGACCGC6800.006056641711.0295397
ACCGCGC10500.00150316239.183788
CGCGTCA3500.0091422937.52472215-219