FastQCFastQC Report
Wed 6 Jul 2016
501725_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501725_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211198
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTTG1750.006206379151.09843235
CCTTTTA255.6475226E-498.84101230-234
GAGCCTC1400.00145365777.82352230-234
TATTGGT1054.7189948E-543.532192
GTATTGG1701.0075382E-843.0200421
CCAATTC2600.002379468635.081696210-214
GTCCAGG4154.8718206E-431.858103235
CTACTAC1752.5784471E-531.3431761
CTATGGC1250.00581117429.253632
CCATAGC1350.00846139427.0866952
CTACACC3150.001381605217.4128744
ACCGATC5301.1340475E-515.5237438
GTTCCAG4306.379179E-414.8819351
GTCCTTG4407.593578E-414.5437086
CTGGCCT4000.006784994613.7126394
TACACCG4100.00798900613.3781845
GTGGTGG10100.004463033313.090211235
GGACCGC5550.004355089311.5301486
ACCGTGC5600.004656554711.4271998
GGCACCG12805.295628E-410.328995235