FastQCFastQC Report
Wed 6 Jul 2016
501725_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename501725_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211198
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAAGA150.007410714142.849322
TAGGTTT450.00205170471.424665
CAGCATA450.00205170471.424669
GTAGGTT701.5360417E-461.2211344
GCGTTAT1100.001426944438.9589041
CCTATGA1350.003896730331.7442931
TCAGGCT1500.0065229328.5698669
GTGTAGA1600.00893766226.7842481
ATCGTGG6350.009461435526.437965235
GTACACG2601.2976369E-424.7239191
GTACGGG2200.001426434824.3493161
ATCCAGG4653.461758E-416.1281471
TGGGGCC4450.00480454514.4454374
AGACCGA4500.005174397514.2849316
ACAGCGC5700.001618509413.1571748
CACCACG11900.00468255638.10279757
CGCACCG16750.00171378917.0358625