FastQCFastQC Report
Wed 6 Jul 2016
500523_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500523_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193148
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTAG100.0035448228182.79134
TTGGGGG1850.005317552158.9955235
TGTAGAA1250.0058167777117.65667235
TCCTACA450.003851295460.9304352
GGGCTGG7950.005447626655.49843235
GCTAGAC500.0058444254.837393
TATGGGG703.3675838E-452.2260862
CCTCTAT550.00851955849.852171
GGGGGGG3154.7602953E-946.68915235
GTGTAGA950.001515586338.4823761
CCCCATG1150.003868690231.7897931
TTGCTAA300.004954185330.4652182
CCTACAC1250.005816777729.2466073
ACCCAGT1759.484836E-426.1130436
AATGGCG2450.006690349418.6521742
TGTTGGT3500.002797374715.6678262
TCCAGGT3650.00369941615.0239422
AAGTGTG750.00444474514.62330415-19
GTTGAAG3850.00527297314.2434781
CCTGGAC4750.001355989713.4688323