FastQCFastQC Report
Wed 6 Jul 2016
500523_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500523_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193148
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG400.0021127313217.13036235
CTGTTAT150.0073133972143.47921
TAATACC400.001263221680.707054
GGTATAT400.001263221680.707051
TCAAATA600.006300918353.8047032
TCCTTCT802.907137E-453.80472
CCTGATA650.00864654949.6658742
CTCGGCA6000.0060678328.950714235
ATGGAGG2059.207386E-426.2461953
GTTCTGG3306.3124223E-419.5653441
ACAGATA708.369402E-418.77300880-84
CCACACC7657.73586E-512.6599284
CATCAAC6000.0023015812.554431
GTCCAGC7950.001514047510.8286191
TCTGTCG1400.007314234510.7609435-39
CCGCCCG12400.00650953367.81035959
ACCGGAA4450.0090410576.664558220-224