FastQCFastQC Report
Wed 6 Jul 2016
500519_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500519_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188308
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATT100.00380704231463.4337235
CGTCCAA1350.0021018288216.805235
TATTGTG350.008450243136.64944210-214
AGAGAGA250.00272753666.48312215-219
AGTAGCA450.00392980160.6201932
CCCTGGG2300.00761381251.2231230-234
AGTAAGC550.00869283249.5983472
TCCGACT1100.003190399433.0655638
GTCTACC1656.9291034E-427.5546361
CTGTGGA1350.0086825126.942312
CTGGGTC3300.00195387216.5327829
GTGTCGT3600.003491151615.1550489
GGGGGGC3000.004184718711.595339210-214