FastQCFastQC Report
Wed 6 Jul 2016
500519_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500519_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188308
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTTA450.003319053186.71089235
GTATTGT450.0020550471.392371
TATTGAA650.00881359149.4254882
ACAGGAG650.00881359149.4254885
GTACCGT1451.2440658E-436.927096
GTGTAGA1200.002191941335.6961861
GTGTACC1350.003904300531.729941
CCTATGA1500.006535579528.5569482
TCCTTGA3352.8040356E-522.3767132
CCGTGTC2500.003005762121.417719
GGATATG1000.00610619113.939588140-144
GTGTTGG6854.522025E-412.5066931
CACCGTG9300.005779389.2119197
AGCAGAT3000.00742918257.7018685185-189