FastQCFastQC Report
Wed 6 Jul 2016
500024_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500024_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180299
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAA150.00610643861154.7128235
TAAGAAA150.00610643861154.7128235
TGTCATT600.007429753142.56685225-229
GCCTTAT450.00468855257.975878
ATATGGC704.302964E-449.69363
TCTGAAA500.00908424149.048946180-184
TCTACGC1050.003160190533.1290663
TGTGCTT1300.00897691726.7580935
CATGCAC1658.9899974E-426.3526653
GTGCTGT2656.0461694E-419.6899191
TCTGGAT1800.006879738518.564184215-219
AAGGCCG3550.004278606314.6981085
GTCCGGG5000.002896169412.1749321
GTGCGCA5050.003120289212.0543889