FastQCFastQC Report
Wed 6 Jul 2016
500024_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500024_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180299
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG150.00849399136.470641
CTATATA150.00849399136.470641
CTATTTA150.00849399136.470641
CGACAAA2500.005066718456.766537235
TGTTAGA550.00544379955.82892
GAAGAGT850.0077502551.050224200-204
GTATGTG1200.002736049234.117661
TTGGACA1653.4654205E-431.0160564
ATTGAGG1350.00487086130.326813
GACAGTG2053.5089182E-529.956977
GCTCAAT1450.0069064928.2353041
CATTGAG1500.00814918627.2941282
GGACAGT1500.00814918627.2941286
TGGACAG2307.68696E-526.7007795
GCTCAGG2550.004380616420.0692141
AAGGTAG800.005952068619.041702210-214
GTACACC2800.00751982718.2773174
GTCTTGG2800.00751982718.2773171
GTGTTGG5450.001627048813.1462551
ACAGGAC1150.002447267512.46036235-39