FastQCFastQC Report
Wed 6 Jul 2016
500005_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500005_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212177
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAA150.0055035021216.6749235
CTAGCTT150.0055035021216.6749235
TATTATT200.009782234912.5062235
TACACGG1156.699373E-4317.39346235
TAGGAGC350.001772835674.1040655
GGGGGGT1700.002728202866.39411220-224
TATTTAC500.007282219451.872852
AAGAGTT500.007282219451.872855
ATAGGAG500.007282219451.872854
GAGATTA200.00923114448.86256155-159
GGCGTTA1854.963255E-448.24456225-229
TTCTGGG2357.124517E-444.823208230-234
ACAGTCC1150.00507869130.0712158
AAAAAAA652.1649612E-829.794981125-129
ACTGGTC2809.1165357E-418.5260168
CTGGCAA2700.00975186917.471972225-229
GGGGGGG3500.002262645416.174051225-229
AGACCGC4355.8948535E-515.8997246
GGGCCGT3600.004885601314.4091246
TGCACCG5050.003263766211.9838285