FastQCFastQC Report
Wed 6 Jul 2016
500005_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename500005_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212177
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAC450.002160566270.49983
CCATATA450.002160566270.49982
GTATAGG854.274502E-449.764571
ATTTAAC150.00925159748.83766160-164
TTACCTC957.3960103E-444.526194
TACGAGT1050.001210362240.2856034
TACTGCC1450.00589079929.1723332
TCATAGG1500.006951634328.1999232
GTGTACG1957.6146726E-427.1153091
CCAGGTA3256.407991E-419.5230224
CCCCCCC750.00844382517.922749195-199
TCAGCCC3750.001674568316.9199524
GCCTTGC4250.003859793514.9293711
GGTACTT1050.00836133913.293121135-139
ACCGTGG7207.65101E-411.7499688