FastQCFastQC Report
Wed 6 Jul 2016
Undetermined_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256803
Sequences flagged as poor quality0
Sequence length50-241
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGAAA550.00221592213.70839235
CTAAGAG150.006051184152.874742
GGATTAG150.006051184152.874741
ACCGAGT450.001568002476.437378
GTGAACG700.00902260149.138311