FastQCFastQC Report
Wed 6 Jul 2016
Undetermined_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256803
Sequences flagged as poor quality0
Sequence length50-241
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGTA150.005935534153.864494
CGTAGCC450.001528352776.932243
ATGTAGG4451.1926168E-554.854965235
GCATTCC650.006561749653.2607843
GAAAATA7050.00754419727.699814235
CTTTAAG5100.00725456513.5762785
TTTAAGC5200.00824851313.3151966
TTAAGCG5300.00935440413.0639667
TAAGCGG5300.00935440413.0639668