FastQCFastQC Report
Wed 6 Jul 2016
MU572163T_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU572163T_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86620
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAACA700.002129409215.94553235
TAACACA700.0098240848.064094
TACACTG1007.105552E-444.8598145
ATACTAT1150.001413420239.0085336
ATAGTGA1350.003104789933.2294928
ATTTAAC1400.00371021732.0427253
CTTATAA1400.00371021732.0427251
ACTGCTT1904.616412E-429.5130358
ATACCTT2057.2004535E-427.3535444
GTCTTTA1650.00828247827.1877671
CAGCACT1000.00515735914.29659175-179