FastQCFastQC Report
Wed 6 Jul 2016
MU572163T_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU572163T_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86620
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAA600.0074158814142.53967235
GGGTTAA650.009420436131.57507235
TATAGAC658.122564E-569.58593
TAGACTG700.00950956548.4616055
GTCTTGT1458.990149E-538.9920921
ATAGACA1752.7162497E-432.307744
GGACCGA300.004469601430.874044120-124
GTATTAT1500.00499478430.1538871
GCCTAAT1500.00499478430.1538871
AACCATC1904.3990207E-429.7571267
GTGTAAC405.958123E-428.285655-59
TACAGCA2450.001940209423.0769544
CTCCGCC706.3246343E-419.56766590-94
CCGTAAA1000.00483546714.436081180-184