FastQCFastQC Report
Wed 6 Jul 2016
BS20102N_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS20102N_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93523
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAGG100.0018398775227.51233
GTATACG150.006189194151.674871
ATGCACG303.2230036E-4113.756144
TGTACAG355.9501175E-497.505275
TGCACGT400.001011512285.3171165
CTAGACC657.8885314E-570.0037844
GTATTAG1255.650454E-754.6029551
CCCTGCC650.00693069752.5028422
TTGTACA1058.4276457E-443.3356744
TTATATA1100.001059596141.365872
TACAGGT1200.001625240837.9187167
AGGACCA1250.001985998836.401975
GTATTAA2309.34926E-734.6214371
CTATTAT3804.6204503E-520.9550821
TATAATA2850.004508804519.957222
TCCAATA3100.00732901718.3477652