FastQCFastQC Report
Wed 6 Jul 2016
BS20102N_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS20102N_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93523
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAG50.00748213941044.7537235
CGTAGTA355.7915214E-498.170132
TAAGGGT904.9700466E-663.6287884
CTTAACG650.00674692452.8608364
ATAGTGC700.00904344849.0850648
CCTTATA1202.7307948E-547.7215921
ATAAGGG1253.4757206E-545.8127253
CTATAAG1100.001024754341.6479341
TTGACTG1500.004693969630.541829
CCTTATG1600.006434910528.6329562
GCCTTAT2107.335554E-427.2694821
TCCAGAA2209.62552E-426.0299592
GTATTAT2250.001097410225.4515151
TATTATG2300.001247459924.8982222
AGGCCTG650.00922765817.64670255-59
ATATTGA4650.00410690214.7782991
GTGAGCA1150.001031351513.97289370-74
CCTTGCC1050.006527765713.797682210-214