FastQCFastQC Report
Wed 6 Jul 2016
607239_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename607239_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166791
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA150.00313665651609.9642235
ACACTAA250.008710564965.9786235
GTATACA350.0042194044172.3423230-234
GTACAGA500.003876564860.8262371
ATGAGTG500.003876564860.8262375
TAGCACC1401.3964028E-436.2060934
TTGAACT1150.002326958335.2615854
GTCTTGT1150.002326958335.2615851
TCTTATA1302.5828194E-729.764938225-229
TCCCTTC700.00143678424.327053215-219
CTCTTAT1401.5427153E-524.184015225-229
TGTCTCT1506.808965E-620.44399220-224
CAGAACC2600.0051790919.4955864
TCTCTTA1000.00572966719.34721225-229
CTGTCTC1409.277547E-519.166243220-224
AGAACCG2850.00879758117.785455
AGGCCCA2850.00879758117.785455
TACCGCA3500.00139811717.3789237
GTCTCTT1602.5783072E-416.770462220-224
TTGTGGG1450.00898639913.147293210-214