FastQCFastQC Report
Wed 6 Jul 2016
607239_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename607239_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166791
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAG150.008091524138.701842
AATAAGT150.008091524138.701843
TGCTACA555.381924E-575.6555562
ATCACAA750.00232078469.21257230-234
TATACCC550.005104726656.741674
CCTAACG600.00720214652.013193
AAAAGAC650.00988202148.0121775
TCTTATA350.003385701232.678574205-209
GTGCTAC1400.00537406129.7218251
CACAACC2300.002191009522.6144314
GCACTGG4600.007267478420.328608235
ACCTTGG2950.009249032517.6315926
TGGCCAG8100.00234202210.2742115
GTGCTGG10952.7214986E-49.5001271