FastQCFastQC Report
Wed 6 Jul 2016
606111_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename606111_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98241
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAA150.00276034841711.5875235
TATTAGA150.00276034841711.5875235
AATTAGG250.00766569381026.9525235
TCTTAAA150.006895697146.28954230-234
CATGTAT150.007959743139.436874
TATACTT150.007959743139.436875
TATACTC150.007959743139.436876
TTTGTCG900.007573501751.321964230-234
CAGAGCG1350.004374922330.985974
AGCCCTA350.00493629230.252865195-199
CTCTTAT851.01164565E-425.651367220-224
CCTGGAC2700.00536685119.3662323
GACTGGG1200.00859209713.235629195-199
GTGCACC2650.00478302048.087567165-169