FastQCFastQC Report
Wed 6 Jul 2016
606111_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename606111_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98241
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTAA50.00281115061703.3279235
ATATTGG500.002968345170.33281235
TCCCTAT150.007147925144.545821
TCAGATT150.007147925144.545824
CGAATGG1250.008438071136.26624235
AATACTG357.2060595E-492.922325
TAGAGCA500.00296834565.045624
ACCTATG600.00611007454.2046852
CCTACTT650.00838491650.0350951
TATTCGA650.00838491650.0350955
AGCAGTC650.00838491650.0350957
CCAACCC2850.005957967619.0191884
GCCAACC3950.001997236416.4672473