FastQCFastQC Report
Wed 6 Jul 2016
506776_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506776_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153749
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAC200.0055185403157.6134220-224
CGGGGGG3709.144353E-5154.84827235
CTTTACA150.00759781141.65042
TGTAATT150.00759781141.65042
GTTCTGG2500.003145484376.39181235
CTAGTGC651.1089335E-465.377113
ATAAGCC500.003219635563.7426763
GGGGGGG3654.405429E-452.32316235
GTCATCT1300.001188767840.4202230-234
GTCCTAC1250.002783079433.9960941
CTGCTCA3706.40442E-520.0990439
AGTCATG655.3679023E-420.05280790-94
ACTGGCC3800.001771220216.774393
TGCTGGA4900.00959684113.008712
GCGGGGG6009.245569E-46.154468210-214