FastQCFastQC Report
Wed 6 Jul 2016
506776_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506776_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153749
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGC150.007086206144.99224
GGCTAGG1250.007630688141.12064235
GCTCCTT1350.00960437130.66725235
TATGGAC400.00121121681.5581053
GTAACTG450.001933028872.496093
CCTACTG853.7211197E-451.1737143
CTACTGC1050.001054129141.4263424
GTACTAC1150.001648624937.8240471
TTCTGGA1150.001648624937.8240472
ACTGTTC1400.00432503131.0697569
TACACCA1450.005134910329.9983832
CTGGAGA2109.959177E-425.8914624
GTGCAGA2150.001142447225.2893351