FastQCFastQC Report
Wed 6 Jul 2016
506683_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506683_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90082
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCA150.0063959161126.4298235
ACTAGGG150.007442178142.601888
GTAAGGG150.007442178142.601883
TAATCCT150.007442178142.601884
TAAGGGC450.002062001771.300944
GGAGCAT550.004567234358.3371356
ATACCAG550.004567234358.3371355
CCAGTAC1300.005822914754.809685230-234
CTAGCCC650.00884342749.3621944
AGGGGGG551.5632587E-435.5262225-229
GTACAGC1250.002687319234.224453
AAGGGCG1550.00769724627.6003655
ATCGGAC1200.00660413213.772307210-214
GGGGGGG2753.7547434E-410.686405220-224
CGGGGGG3100.00298704658.519468215-219