FastQCFastQC Report
Wed 6 Jul 2016
506683_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506683_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90082
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGT600.005761832555.0109671
TACACGC650.00790745150.7793582
TTGCACG1150.001553247438.2684974
AATCACC1350.003410964532.599096
TCCAGGA1803.7636887E-430.561652
CTACACC1803.7636887E-430.561654
ATGCTGC1600.00782768427.5054825
CCCTGGG1600.00782768427.5054828
TGGACCG3150.00973864817.4637995