FastQCFastQC Report
Wed 6 Jul 2016
505907_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505907_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197665
Sequences flagged as poor quality0
Sequence length50-241
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGG1300.008343066137.04874235
CCCTATA1250.002398844335.046762
GTACGGC1550.00687447828.2635151
TGCACCG3554.2609827E-827.7659195
GTCTTAT2109.5635676E-426.0764581
ATACTGT2400.002081746522.81696
ACCTTTG2952.6300532E-422.2754827
TTGATCT2750.004584885719.9129314
GTGCACC3356.2047667E-419.6157254
TAAAGAC3050.00833678817.9542834
ACCGGTC4250.003055721515.4618058
ATATTAG4600.00517180914.2853641
TACCTTT5459.768513E-414.0669336
GTTATAT5050.00959153413.0124111
CTTTTAA7608.969783E-411.721275235
TCTATAT7558.486941E-411.6048873
CTATATT6950.006054918311.0309054
ATATAAT10751.3483132E-410.1880121
ACGTGGT2600.0041380688.4247021
TATAATA11900.00383785328.283112