FastQCFastQC Report
Wed 6 Jul 2016
505137_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505137_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83440
Sequences flagged as poor quality0
Sequence length50-241
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC651.0231596E-466.413213
GCCCCCT800.004795335257.598015220-224
TACTCCG650.00853125149.8099065
TCTGGAC1150.001706794637.53793
TAACAGC350.00882246226.8611165-169
GATGCCC2750.00116512378.217333135-139