FastQCFastQC Report
Wed 6 Jul 2016
505137_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename505137_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83440
Sequences flagged as poor quality0
Sequence length50-241
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG100.0019707219222.335721
TTACACC207.024485E-5166.75184
TTAACCT251.7088215E-4133.401434
TCCCAGA450.001768306274.111912
TACAGGT650.00758813951.308258
TACGGCC1600.007438627.7919654
ACGGGTA350.0089302526.7945145-149
GGTGAAC2450.002141466122.687323
GGGGGGG1050.00575888314.060358195-199