FastQCFastQC Report
Wed 6 Jul 2016
504782_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504782_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144769
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTAAA55.61152E-43808.736235
GAGTTTA150.00504862141269.5789235
AATTTAA150.0048111426165.025230-234
GGGGGGG3650.00472776104.34894235
GCTTACT500.003103572264.33411
CGTTAAG600.00638787753.6117442
CTACGGT1501.505704E-435.7411654
TACGGTG2008.1301667E-426.8058745
GGTCATG2950.00776379618.1734731
TGTCTCT850.00999193717.404945215-219
TGCTGGA4300.00379735814.9614172
ACACCGA4950.00965154612.9967866
CAACACC8150.001913858110.5250064
GCACCGC11600.00367852328.3190646