FastQCFastQC Report
Wed 6 Jul 2016
504782_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504782_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144769
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGC150.0069025643146.268281
ATTCATA550.00413554559.8370252
TACATGC600.00583590854.8506052
CTACACA650.00800900250.631332
TGTAGAG650.00800900250.631332
CTCCAGG1400.00414269831.34321
GTGTCAG1400.00414269831.34321
TCCAGGA2552.792236E-630.1140562
TACTGGG1650.00924392626.5942332
CTCGTAA550.002889878321.573164160-164
CCAGGAC3406.768117E-419.3590373
ACCAGCG7350.00904341210.4477347