FastQCFastQC Report
Wed 6 Jul 2016
504302_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504302_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211816
Sequences flagged as poor quality0
Sequence length50-241
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTT200.00618110881147.6973235
GGTTTTT1406.0653465E-4327.91348235
CTAGGAC400.00186600573.15353
TTGTACT550.00658861653.202552
CGCCCGG13350.00721758151.581898235
ATAAGAA700.00827485850.219307230-234
CCCTTAC600.00929335648.7690051
GGGGGGG5301.0280928E-743.30933235
AATACCT1553.1879198E-431.4638755
GAGCAAA1900.001268036324.846712230-234
CAATACC2602.4518254E-422.508774
CTGTCTC2150.001120193521.75128225-229
TCGTGGA3406.462699E-520.0813542
AGGGGGG1100.00551188219.295694220-224
TTCACGC2650.00723156918.4033992
CTCTTAT2100.003697186514.756107225-229
CAATTCA1150.007005889413.653017170-174
CGTGGAC4350.00769793513.4535193
TCTTATA1600.00847249213.26579220-224
ATCTGAC2905.3587915E-513.174302220-224