FastQCFastQC Report
Wed 6 Jul 2016
504302_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename504302_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211816
Sequences flagged as poor quality0
Sequence length50-241
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCATG950.0025631536203.30049235
ACCTCTT1250.0058254506154.50838235
GAAGATT1300.006550315148.56575235
GACTATA150.009210077132.830031
TAGACCG804.2547547E-449.811265
GTTATTG1100.00203951636.226372
TAGAACG1300.004624281530.6530842
ACAGATC2650.006400793318.7967018
ACACCTG2800.00879300617.7897345
CTGGGCT3650.002083236816.3763054
GCCCAAG3800.002725784715.7298731
GCACTGG4550.00900935613.1370351
TTTTTTT1850.00314915412.047386205-209
CAGGTCG11500.0066189837.79654464
GAACAGC4950.00478737575.816321145-149