FastQCFastQC Report
Wed 6 Jul 2016
503710_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename503710_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92160
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTTT54.0794665E-44455.3296235
TACGCTA150.0036704461485.1099235
GCCTATA200.00652428341113.8324235
CCATAGA150.007565357141.822751
CAATTGA403.2736972E-4113.27109230-234
GGGGGGG3100.00169908971.86015235
CGTATCA500.003826472261.006588225-229
TCTCCGT550.00466772358.0184026
CTTGGAT650.00903763249.0924873
GTTGTGA650.00903763249.0924876
CCTTGGA1350.00402506331.5161672
CCAGTGC2150.001295600724.7365288
CGCGGGG3600.0020055948.9009495220-224