FastQCFastQC Report
Wed 6 Jul 2016
502836_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502836_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133949
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAA100.00253740861790.9166235
ATGGAGG1157.0631655E-4311.46378235
GACAGAA350.00842903627.11608175-179
CCCCCCC852.7970384E-522.396826210-214
ATGACCG4955.099677E-415.320495
CCAGCAC7950.001426218910.9019123