FastQCFastQC Report
Wed 6 Jul 2016
502836_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename502836_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133949
Sequences flagged as poor quality0
Sequence length50-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTTA50.00363221561499.5703235
TGTCTGG900.004645431166.61894235
CAGGAAA1000.0063660154149.95703235
TTATGAT150.0065864217148.57584
TAGGAAC400.001098986183.573892
GGACGGG2950.001561920376.24934235
TGTAGGG958.077886E-658.6483422
ACATGCA650.007527219651.4300842
GTAGGGC904.371532E-449.525273
CTACATC1550.00631048928.7566074
TGGACTG1650.00856236127.013785
GGACTGG3201.4425279E-524.3757176
ACCGCGC7752.2584754E-715.8161348
AGCACCG6502.0668603E-413.714695
GCACCGC9353.6005382E-410.726066
CACCGCG12755.1286606E-610.4877037
CCGCGCC11602.4260732E-49.60619459
CCGCGCG9600.00539360249.2859889
CGCATTC2050.0093149358.69712135-39